C.B.S. Scientific DGGEK-2401 - Manual (Page 26)

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Cipher DGGE Instructions 2/28/14
4 2 Buffer Cycling Connections-continued
Buffer Cycling Connections – Dual Cassettes
SECTION 5
Running Conditions
5 1 Running the Gels
1. Load samples at 1:1 with neutral dye. Load 5-10 ug Genomic DNA/well or 1-2 ug
cloned (B-globin)/well. Determine concentration by O.D. 260.
2. Attach black power leads to cassettes as shown in Fig. 5-1.
3 Close lid to engage safety interlock Turn on power supply to 150V (40mA)
constant Volts for 5-7 hours.
Fig 5-1
Fig 4-2e