C.B.S. Scientific DGGEK-2401 - Manual (Page 25)

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4 2 Buffer Cycling Connections
1. Fill upper reservoir(s) by attaching recirculating tubing to barb fitting on each
upper reservoir (fig. 4-2c,d,e). Rinse loading interface of gel with tank buffer
to remove non-polymerized acrylamide or excess urea. Adjust final level of
lower reservoir buffer by filling the tank until the level of buffer reaches the
underside of the upper reservoir.
Buffer Cycling Connections – Two Single Cassettes
Fig 4-2c
Fig 4-2d