C.B.S. Scientific DGGEK-2401 - Manual (Page 18)

18
Cipher DGGE Instructions 2/28/14
3 2 Gel Casting Techniques- continued
B. Vertical gradient gel casting using GM-40 gradient maker and gravity flow.
1. Place the GM-40, gradient maker, on an elevated magnetic stirrer with a small
“flea” stir bar in the cylinder (C-2) closest to the outlet. The gradient maker
should be fitted with a leur valve, (V-2) and a 20ga needle with attached tubing
to deliver acrylamide to the gel plate sandwich as shown in figure (3.5). With
the height differential between the leur valve (V-2) and the top of the gel plate
sandwich between 2-4 inches, it takes approximately 5-9 minutes to cast the
gel. Raising the height of the gradient maker can increase flow rate. Flow can
be regulated by turning leur valve (V-2). Flow rate appropriate for each gradient
should be determined empirically.
Fig 3-5
C-1
C-2
V-1
V-2