C.B.S. Scientific DGGEK-2401 - Manual (Page 7)

7 www.cbsscientific.com
Figure 2-1
Heater
Stirrer
Buffer Siphon
Pump Assembly
Vapor
Shield
Thermometer
DGGE
Tank
Single
Cassetes
Comb (2)
Spacers
(2 pairs)
Glass Plate
Assembly(2)
Clamps
Mini-Pump
(Optional)
GM-40
Gelwrap ® (2)
Buffer recycling
manifold
2. Open the lid and confirm the following:
a) Two lengths of small bore silicone tubing for buffer cycling are already attached
to the buffer recycling manifold outlets
b) A single length of large bore tubing from the By-Pass Pump is connected to the
barb-fitting on the plastic manifold.
DGGEK-2001
2 2 Filling DGGE tank with Buffer
1) Fill the tank with running buffer (see Section 6 for buffer formulations) to optimal
level for number and type of cassettes used. Refer to chart below for exact buffer
volumes required: This requires 20 to 21.2 liters of buffer. See chart below:
Use distilled or RO water when making buffer
NOTE: Buffer Displacement Tank Instructions:
With DGGE-1001 - fill buffer displacement tank, BDT-1000 with water and place in
the back of DGGE tank.
With DGGE-2001 or DGGE-2401- If using only 1 cassette, BDT-1000 can also be
used to save buffer but must be ordered separately.
Model Cat. # Cipher DGGEK-1001 Cipher DGGEK-2001 Cipher DGGEK-2401
Optimal Buffer
Volume
21 Liters -1 dual cassette 21.5 Liters - 1 single cassette
21 Liters - 2 single cassettes
21 Liters - 1 dual cassette
20 Liters - 2 dual cassettes