Leica Biosystems ST5010 Autostainer XL - Manual (Page 32)

Instructions for Use V 2.3 – 10/2012 32
5.9.1 Reagent containers
Reagent containers can be individually removed for filling. For use, fill the
reagent containers to the line marked on the inside (450 ml capacity) and
place into position in the instrument consistent with the programs you wish
to run.
There is an area for a label on the end of the containers just above the handle
pivots.
The container map inside the instrument (see Figure 11) defines the station
numbers. Ensure that the reagent containers are correctly seated and that
the handles are over to the side and will not obstruct slide rack movement.
Lids are provided to reduce evaporation while the reagent containers are
not in use.
The Load and Exit drawer containers can be filled with a reagent if desired.
However, the instrument will not control the immersion time in these sta-
tions.
5.9.2 Wash system
The wash system consists of five wash stations each capable of holding
one slide rack. Water enters the wash station from the base and exits from
the overflow lip at the top left hand edge.
Wash stations have a locating pin and can only be inserted
one way. Take care when fitting or removing wash stations
as the seals may be damaged by excessive force. Wet the ‘O’
ring seal before fitting a wash station.
To use the wash system, slowly turn the laboratory tap on fully. The flow
control valve in the AutoStainer XL will limit the total water flow in the wash
stations to 8 liters/minute.
If the water flow drops below this level for any reason the
wash period specified in the program may have to be ex-
tended.
5. Operation