Appendix 3, Compatible staining programs – Leica Biosystems ST5010 Autostainer XL User Manual
Page 46
Instructions for Use V 2.3 – 10/2012
46
Reagent
Station
Step
Time
Exact
Step
Time
Exact
Oven
1
10:00
Y
Xylene
1
2
2:00
N
Xylene
2
3
2:00
N
100% Alcohol
3
4
2:00
N
100% Alcohol
4
5
2:00
N
70% Alcohol
5
6
1:00
N
1
1:30
N
Wash
Wash 1
7
2:00
N
2
2:00
N
Hematoxylin
6
8
5:00
Y
3
3:30
Y
Wash
Wash 2
9
2:00
N
4
2:00
N
Acid Alcohol
7
10
0:02
Y
5
0:05
Y
Wash
Wash 3
11
3:00
N
6
2:00
N
Scott’s
8
12
3:00
Y
7
4:00
Y
Wash
Wash 4
13
3:00
N
8
2:00
N
95% Alcohol
9
9
1:30
N
OG 6
10
10
2:00
Y
95% Alcohol
11
11
1:30
N
95% Alcohol
12
12
1:30
N
EA 50
13
13
2:30
Y
Eosin
14
14
2:00
Y
95% Alcohol
15
15
0:30
y
14
1:30
Y
100% Alcohol
16
16
2:00
N
15
1:30
Y
100% Alcohol
17
17
2:00
N
16
1:30
Y
100% Alcohol
18
18
2:00
N
17
1:30
y
Xylene
Exit
19
18
Washes 1 to 4 (and the stations between) are used in the same sequence in both pro-
grams. - These programs are compatible with each other but not with programs on
page 47.
APPENDIX 3
Program 1H&E
Program 2 Papanicoleau
Compatible staining programs