Metrohm 817 Bioscan - Manual (Page 68)

5 Notes – Maintenance – Faults
817 Bioscan
60
5.2.4 Changing separating columns
Identical separation system
If you wish to replace an IC separating column by a column of the same
type, proceed as follows (see Fig. 3):
1 Remove old column
• Switch off flowcell.
• Switch off pump drive of the 709 IC Pump.
• Unscrew connection capillary 21 to flowcell from the column.
• Unscrew preheating capillary 31 from column.
2 Connect new column to injector
• Remove end caps from column.
• Screw preheating capillary 31 to inlet end of separating
column (note flow direction).
3 Rinse column
• Place beaker beneath the column outlet.
• Switch on 709 IC Pump and rinse column with eluent for ca.
10 min with 0.3 mL/min and for ca. 50 min. with 1.0 mL/min,
then switch off pump.
4 Connect column to flowcell
• Screw connection capillary 21 to flowcell to outlet end of
separating column.