Priming and testing the pump, Tubing connections (m6 shown), Initial priming – VICI M Series User Manual
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FITTING DETAIL
PILOT
TUBE
NUT
FERRULE
Priming and Testing the Pump
At this stage, some basic tests will determine that the tubing connections are
tight and that the pump is rotating in the proper direction. The tests include an
initial priming of the pump to remove all air and unwanted solutions in the tubing.
Use proper safety precautions when pumping flammable
liquids. In all applications, make sure you have enough
tubing and sufficient quantities of solutions ready for
use before beginning a procedure.
Tubing Connections (M6 shown)
Improperly installed fittings can create space for a bubble
to become trapped, degrading pump performance.
M6 pumps should not be used with tubing ODs smaller
than 1/16". For M50 pumps, the minimum OD is 1/8".
1. Connect a length of tubing to the inlet (Port A) and place the other end in
a vessel containing isopropyl alcohol.
2. Connect a second length of tubing to the outlet (Port B) and place the
other end into a receiver bottle.
Fitting Assembly Instructions
1. Slide the nut and ferrule onto
the tubing in the order shown.
2. Insert this assembly into the
fitting detail, screwing the nut in
two or three turns by hand.
3. Push the tubing all the way forward into the detail so that it seats firmly.
This is essential for a proper zero dead volume connection.
4. Turn the nut into the detail until it is finger tight.
Initial Priming
In the Test Mode, a mouse click on a button directs the pump to either
aspirate or dispense. (Refer to page 13, No. 7 for a definition, if required.)
When the Enable box in the Fixed Volume area is checked, (see
Figure 8)
the pump will aspirate or dispense the quantity entered in the Fixed Volume
field and then stop. If the Enable box is not selected, the pump will continue
to run at the set Flow Rate until either Stop (stops aspirating or dispensing)
or Quit (exits the Test Mode) is selected.
If the pump aspirates a volume larger than the outlet
tube volume, sample or reagent could enter the pump
and mix with liquid in the reservoir.