Bio-Rad PDS-1000 / He™ and Hepta™ Systems - Manual (Page 23)

Consumable Preparation
Several consumables are available for use with the PDS-1000/He system (Figure 4.3).
Fig. 4.3. Consumables for the PDS-1000/He instrument. The 0.6 micron gold is not pictured.
1. Macrocarriers
Pre-assemble and pre-sterilize the macrocarrier set in a macrocarrier holder prior to per-
forming sample cell/tissue bombardments. The Disk-Vac (catalog number 165-2323)
provides ease in handling rupture disks and macrocarriers.
Macrocarriers are shipped in quantities of 500/box, with paper linings between disks.
Transfer selected macrocarriers to individual Petri dishes for easier handling. Remove
the paper lining from between the macrocarriers. Place the macrocarrier into the macro-
carrier holder using the seating tool (Figure 4.4). The edge of the macrocarrier should be
securely inserted under the lip of the macrocarrier holder. The macrocarrier holders, with
macrocarriers already in place, should be sterilized by autoclaving.
2. Rupture disks
Rupture disks (Figure 4.3) are packaged and shipped in quantities of 100/box. Transfer
selected rupture disks to individual Petri dishes for easier handling. Sterilize rupture disks
by briefly dipping them in 70% isopropanol just prior to insertion in the Retaining Cap.
Do not soak for more than a few seconds. Extensive soaking may delaminate the disks,
resulting in premature rupture. All disks, with the exception of those rated at 450, 650, and
1,100 psi, are laminated. Autoclaving is not recommended because of potential delami-
nation.
20
A. Macrocarriers
B. Rupture disks
C. Stopping screens
D. Tungsten microcarriers
E. Gold microcarriers
A
C
D
E
B