Bio-Rad PDS-1000 / He™ and Hepta™ Systems - Manual (Page 28)

7. Loading the rupture disk
Unscrew the rupture disk retaining cap from the gas acceleration tube from within the
bombardment chamber (Figure 4.6) or unwrap cap from sterile wrapping.
Fig. 4.6. Removal/mounting of rupture disk retaining cap onto end of gas acceleration tube inside
bombardment chamber.
Select rupture disk of desired burst pressure. Handle all rupture disks with sterile forceps
or Disk-Vac (catalog number 165-2323). Grease, fingerprints, or even powder from plastic
gloves on the rupture disk may prevent a tight seal from forming within the retaining cap.
Fig. 4.7. Rupture disk insertion into recess of retaining cap.
Immediately before placing a rupture disk in the retaining cap, briefly wet the rupture
disk in isopropanol (do not soak the disk for an extended period of time or the disk may
delaminate). Loading the rupture disk while wet forms a liquid gasket when the cap is screwed
onto the gas acceleration tube, and thereby reduces the failure rate of the rupture disk. Place
the rupture disk in the recess of the rupture disk retaining cap (Figure 4.7).
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