Bio-Rad Criterion™ XT Tris-Acetate Precast Gels - Manual (Page 10)

Section 3
SDS-PAGE and Native PAGE
3.1 Criterion XT Gel Selection Guide
Criterion XT gels are available in a wide selection of single acrylamide percentages and gradients for the
separation of proteins by SDS-PAGE or native PAGE.
Optimal Separation
Bis-Tris Gels With XT MES Running Buffer With XT MOPS Running Buffer
10% 2.5–200 kD 14–220 kD
12% 1–30 kD 6–66 kD
4–12% 2.5–200 kD 10–300 kD
Tris-Acetate Gels* With XT Tricine Running Buffer With Tris/Glycine Running Buffer
7% 36–200 kD N/A
3–8% 40–400 kD N/A
*Because Criterion XT Tris-acetate gels are made without SDS, they can be used to separate proteins by both SDS-PAGE and native PAGE.
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