Bio-Rad Criterion™ XT Tris-Acetate Precast Gels - Manual (Page 8)

Section 2
Setup and Basic Operation
2.1 Setting Up and Running Criterion XT Gels
1. Each Criterion XT gel is packaged individually in a plastic storage tray. Remove the cover by gently pulling
the corner tab up and diagonally across the package. Remove the gel from the package.
2. Remove the comb and gently rinse the wells with ddH
2
O or running buffer.
3. Remove the tape from the bottom of the cassette by pulling the
tab across the gel.
4. Insert the Criterion XT gel into one of the slots in the Criterion cell
tank. Ensure that each integral buffer chamber faces the center of
the cell.
5. Fill each integral buffer chamber with 60 ml running buffer.
6. Load samples using a Hamilton syringe or a pipet with gel loading
tips. A sample loading guide can be placed on the outer edge of
the cassette to aid in aligning pipet tips with the wells. This is
especially useful with multichannel pipets.
7. Fill each half of the lower buffer tank with 400 ml of running buffer
to the marked fill line.
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