Bio-Rad Criterion™ XT Tris-Acetate Precast Gels User Manual
Page 6
complexes. Separation by native PAGE with XT Tris-acetate gels uses discontinuous acetate and glycine
ion fronts to form moving boundaries to stack and separate proteins by both size and charge.
Protein samples for the Criterion XT precast gel system are prepared in a reducing denaturing sample buffer.
The sample buffer contains XT reducing agent, a pH neutralized and stabilized solution of TCEP as the
reducing agent; heat and SDS are used to denature the proteins. In addition, the use of TCEP in combination
with Bio-Rad’s optimized running buffers maintains proteins in a fully reduced state during the electrophoresis
run, eliminating the need for an anti-oxidant in the upper buffer chamber. Criterion XT Tris-acetate precast
gels can also be used for native PAGE. Proteins are prepared in a nonreducing, nondenaturing sample buffer,
which maintains the proteins’ native structure and charge density.
3
4110130B.qxd 3/25/2003 2:19 PM Page 3