Bio-Rad Criterion™ XT Tris-Acetate Precast Gels User Manual

Page 9

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8. Place the lid on the tank, aligning the color-coded banana plugs and jacks. See section 3.6 for power

conditions.

2.2

Opening Criterion XT Cassettes and
Removing the Gels

1. After electrophoresis is complete, turn off the power supply

and disconnect the electrical leads.

2. Remove the lid from the tank and remove the Criterion XT

gel(s) from the cell. Pour off and discard the upper running
buffer.

3. Invert the cassette and place the integral buffer chamber over

the cassette-opening tool built into the Criterion cell lid.

4. Firmly press down on the cassette to crack the cassette welds

on both sides of the cassette. The cassette will split open
approximately 1/3 of the way.

5. Alternatively, the gel cassette can be opened by sliding the

tapered back of the comb into the slits on either side of the
cassette.

6. Pull the two halves of the cassette apart to completely expose

the gel.

7. Remove the gel by either floating the gel into a fixing or staining

solution or by carefully lifting the gel from the cassette.

6

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