Bio-Rad Criterion™ XT Tris-Acetate Precast Gels User Manual
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Table of Contents
Section 1 General Information.............................................................................................. 1
1.1 Introduction........................................................................................................................................... 1
1.2 Criterion XT Precast Gels...................................................................................................................... 2
1.3 Criterion System Specifications............................................................................................................. 4
1.4 Criterion XT Comb Configurations......................................................................................................... 4
Section 2 Setup and Basic Operation................................................................................... 5
2.1 Setting Up and Running Criterion XT Gels.............................................................................................5
2.2 Opening Criterion XT Cassettes and Removing the Gels...................................................................... 6
Section 3 SDS-PAGE and Native PAGE................................................................................. 7
3.1 Criterion XT Gel Selection Guide........................................................................................................... 7
3.2 Bis-Tris Gel Composition...................................................................................................................... 9
3.3 Tris-Acetate Gel Composition............................................................................................................... 9
3.4 Criterion XT Buffers and Reagents........................................................................................................ 9
3.5 Sample Preparation..............................................................................................................................10
3.6 Running Conditions .............................................................................................................................10
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