Bio-Rad Criterion™ XT Tris-Acetate Precast Gels - Manual (Page 19)

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Problem Possible Cause Solution
Vertical streaking Samples overloaded Dilute sample
Selectively remove predominant protein in
the sample
Sample precipitation Centrifuge samples to remove
particulates prior to sample loading
Gels run too fast, provide poor resolution, Running buffer is too concentrated Check buffer composition
and gel temperature is too high
Artifact bands at ~60–70 kD Possible skin keratin contamination Wear gloves while cleaning all dishware
and while handling and loading gel
Filter all solutions through nitrocellulose
Use 10% iodoacetamide to eliminate
keratin bands
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