Bio-Rad Criterion™ XT Tris-Acetate Precast Gels - Manual (Page 14)

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Section 4
2-D Electrophoresis
4.1 Equilibration
Use existing equilibration protocols as described in the ReadyPrep 2-D Starter Kit (catalog #163-2105 or
bulletin 411009) or existing protocols and buffers used for Tris-HCl gels.
4.2 Agarose Overlay
Make a solution of 0.6% low melt agarose and 0.002% Bromophenol blue. To make 10 ml of the agarose
overlay, mix 9.5 ml of the above agarose with 0.5 ml of 20x XT Running Buffer. Use the XT Running Buffer that
will be used to run the second dimension gel.
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