Bio-Rad Criterion™ XT Tris-Acetate Precast Gels - Manual (Page 12)

3.2 Bis-Tris Gel Composition
Gel buffer Bis-Tris.HCl, pH 6.4
Cross linker 5% C
Stacking gel 4% T, 5% C
Storage buffer Bis-Tris.HCl, pH 6.4
Shelf life 12 months; individual expiration date is printed on each cassette; store flat at ambient temperature
3.3 Tris-Acetate Gel Composition
Gel buffer Tris-acetate, pH 7.0
Cross linker 3.8% C
Stacking gel 4% T, 3.8% C
Storage buffer Tris-acetate, pH 7.0
Shelf life 8 months; individual expiration date is printed on each cassette, store flat at 4°C
3.4 Criterion XT Buffers and Reagents
Bis-Tris running buffer 20x XT MOPS (dilute to 1x) or XT MES (dilute to 1x)
for SDS-PAGE For separation of mid-size proteins For separation of small proteins
Catalog #161-0788 Catalog #161-0789
Tris-acetate running buffer 20x XT Tricine (dilute to 1x)
for SDS-PAGE For separation of large proteins
Catalog #161-0790
Tris-acetate running buffer 10x Tris-Glycine (dilute to 1x)
for Native-PAGE Catalog #161-0732
XT sample buffer Catalog #161-0791
XT reducing agent Catalog #161-0792
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