Bio-Rad Mini Trans-Blot® Cell - Manual (Page 19)

Mini-Trans-Blot Electrophoretic Transfer Cell 13
Voltage limits
Do not increase voltage settings beyond those indicated
in Table 3.1. If overnight transfers at low voltages are
ineffective for your application, and higher voltages are
necessary, transfer times must also be decreased. Failure
to do so may result in a potential safety hazard.
3.3 Buffer Formulation
All formulas provided below are for a total volume of 1 L of
buffer. Approximately 950 ml of buffer are required for the
Mini Trans-Blot cell with cooling unit. Ethanol can be used
in place of methanol in all buffer formulations.
Do not add acid or base to adjust pH of the following
buffers. Methanol should be analytical reagent grade, as
metallic contaminants in low grade methanol will plate on
the electrodes.
Note: Some pH electrodes will not perform a proper measurement for
the pH of Tris buffers. If the pH of the buffer is off, check to make sure
the electrode is designed to work with Tris buffers. If the pH electrode
functions properly for Tris buffers and the pH is below 8.0, remake the
buffer.
25 mM Tris, 192 mM glycine, 20% v/v methanol, pH 8.3
Mix 3.03 g Tris, 14.4 g glycine, and 200 ml of methanol;
add distilled deionized water (ddH
2
O) to 1 L.
25 mM Tris, 192 mM glycine, pH 8.3
Mix 3.03 g Tris and 14.4 g glycine; add ddH
2
O to 1 L.
48 mM Tris, 39 mM glycine, 20% v/v methanol, pH 9.2
Mix 5.82 g Tris and 2.93 g glycine in ddH
2
O, add 200 ml
methanol.
Add to 1 L with ddH
2
O.
48 mM Tris, 39 mM glycine, pH 9.2
Mix 5.82 g Tris and 2.93 g glycine.
Add ddH
2
O to 1 L.