Bio-Rad Mini Trans-Blot® Cell - Manual (Page 27)

Mini-Trans-Blot Electrophoretic Transfer Cell 21
Table 5.1 Guide to Protein Blotting Membranes
Membrane Pore Size Binding
Capacity
(μg/cm
2 )
Notes
Nitrocellulose 0.45 μm
0.2 μm 80–100 General purpose protein blotting
membrane.
Supported
Nitrocellulose 0.45 μm
0.2 μm 80–100 Pure nitrocellulose cast on an
inert synthetic support; increased
strength for easier handling and
for reprobing.
PVDF 0.2 μm 170–200 High mechanical strength and
chemical stability, used for protein
sequencing and western blotting;
enhanced binding in the presence
of SDS. Must be wet in alcohol
before equilibration in buffer.
Nylon 0.2 μm 170 Recommended for nucleic acids.
Note: Nucleic acids cannot be transferred to nitrocellulose by electrophoretic
blotting. Use Zeta-Probe membrane.