Bio-Rad Mini Trans-Blot® Cell - Manual (Page 12)

6 Mini-Trans-Blot Electrophoretic Transfer Cell
2.2 Preparation for Blotting
Store the blue cooling unit in your laboratory freezer at
–20°C until ready to use. After use, rinse the outside
container with water and return the cooling unit to the
freezer for storage.
1. Prepare the transfer buffer. (See Section 3.3 for buffer
formulation. Using buffer chilled to 4°C will improve
heat dissipation.)
2. Cut the membrane and the filter paper to the
dimensions of the gel or use precut membranes
and filter paper. Always wear gloves when handling
membranes to prevent contamination. Equilibrate the
gel and soak the membrane, filter paper, and fiber
pads in transfer buffer (15–20 min depending on gel
thickness).
3. Prepare the gel sandwich.
a. Place the cassette, with the gray side down,
on a clean surface.
b. Place one prewetted fiber pad on the gray
side of the cassette.
c. Place a sheet of filter paper on the fiber pad.
d. Place the equilibrated gel on the filter paper.*
e. Place the prewetted membrane on the gel.*
f. Complete the sandwich by placing a piece of
filter paper on the membrane.*
g. Add the last fiber pad.