Bio-Rad Mini Trans-Blot® Cell - Manual (Page 15)

Mini-Trans-Blot Electrophoretic Transfer Cell 9
8. Put on the lid, plug the cables into the power supply,
and run the blot. Refer to Section 3 for run times and
voltage settings with various buffers.
9. Upon completion of the run, disassemble the
blotting sandwich and remove the membrane for
development. Clean the cell, fiber pads, and cassettes
with laboratory detergent and rinse well with deionized
water.
2.3 Acidic Transfers
If transferring under acidic conditions, switch the gel and
membrane in the set up instructions. This will place the
membrane on the cathode side of the gel. Under acidic
conditions, proteins will transfer in the opposite direction
going toward the negative cathode.