Bio-Rad Mini Trans-Blot® Cell - Manual (Page 5)

Table of Contents
Assembly and Disassembly .......................................... i
Wash Cell Before Use ................................................... i
Warranty ........................................................................ i
Section 1 Introduction .................................................. 1
1.1 Specifications ................................................. 3
1.2 Safety Instructions .......................................... 4
Section 2 Mini Trans-Blot Cell Assembly and
Preparation for Transfer ............................................... 5
2.1 Mini Trans-Blot Cell Description and
Assembly of Parts .......................................... 5
2.2 Preparation for Blotting .................................. 6
2.3 Acidic Transfers ............................................. 9
Section 3 Transfer Conditions ....................................10
3.1 General Guide to Transfer Buffers and
Running Conditions.......................................10
3.2 Notes on Electrophoretic Transfer
Conditions ....................................................11
3.3 Buffer Formulation ........................................13
Section 4 Strategies for Optimizing
Electrophoretic Transfer ............................................. 15
4.1 Optimizing Protein Transfer ...........................15
4.2 Optimizing DNA and RNA Transfer................ 18
Section 5 Choice of Blotting Membranes.................. 19
5.1 Protein Blotting ............................................ 19
5.2 DNA and RNA Blotting Membranes ............. 20
Section 6 Troubleshooting Guide .............................. 22
6.1 Electrophoretic Transfer ............................... 22
Section 7 References ................................................. 27
Section 8 Product Information .................................. 29