Bio-Rad Mini Trans-Blot® Cell - Manual (Page 20)

14 Mini-Trans-Blot Electrophoretic Transfer Cell
10 mM NaHCO
3
, 3 mM NaCO
3
, 20% methanol, pH 9.9
Mix 0.84 g NaHCO
3
and 0.318 g NaCO
3
in ddH2O, add
200 ml methanol.
Add to 1 L with ddH
2
O.
1.0x TBE (Tris-Borate EDTA), pH 8.3
90 mM Tris-Borate, 1 mM EDTA
5x stock solution
54 g Tris base
27.5 boric acid
20 ml 0.5 M EDTA (pH 8.0)
Add 200 ml 5x stock solution to 800 ml ddH
2
O to make
1x working solution.
1x TAE (Tris-Acetate EDTA)
40 mM Tris-Acetate, 1 mM EDTA
50x stock solution
242 g Tris base
57.1 ml glacial acetic acid
100 ml 0.5 M EDTA (pH 8.0)
Add 20 ml 50x stock solution to 980 ml ddH
2
O to make
1x working solution.