Bio-Rad Mini Trans-Blot® Cell - Manual (Page 28)

22 Mini-Trans-Blot Electrophoretic Transfer Cell
Section 6
Troubleshooting Guide
6.1 Electrophoretic Transfer
Poor electrophoretic transfer (as detected by staining
the gel)—proteins
1. Transfer time is too short.
• Increase the transfer time
2. Power is too low.
• Always check the current at the beginning
of the run. The current may be too low for a
particular voltage setting. If the buffer is prepared
improperly, the conductivity may be too low, and
not enough power will be delivered to the cell.
See the power guidelines for specific applications
in Section 3
• Remake the buffer or increase the voltage
• Try the high intensity blotting option
3. Power supply circuit is inoperative, or an inappropriate
power supply was used.
• Check the fuse. Be sure the voltage and current
output of the power supply match the needs of
the blotting instrument
4. Transfer apparatus is assembled incorrectly, and the
proteins are moving in the wrong direction.
• The gel/membrane sandwich may be assembled
in the wrong order or the cassette is inserted in
the tank facing the opposite orientation. Check
the polarity of the connections to the power sup-
ply
• Use a pre-stained protein standard to assess
transfer efficiency after blotting