Bio-Rad Components for Older Model Spot Cutter - Manual (Page 19)

8. If any of the wells are empty and you want to re-cut those spots, click on “Specify re-cuts.”
Then follow these on-screen instructions:
Fig. 23. Message window before recuts are started.
9. Return the microtiter plate to the plate holder.
10. Remove the plastic sheet from the top of the gel.
11. Do not move the gel. The image and spot selections that were already made still apply to
the gel as long as it does not move.
12. After removing the microtiter plate, click “OK” on the message. The "Image Microtiter
Plate" window will close and an image of the microtiter plate will open in front of the Basic
Excision Tool screen. If you need to resize the window, close the recuts box, resize the
window, make any zoom or transform changes using the icons on the top bar above the
window, then click on the "Recuts" icon to bring the recuts box back up.
Fig. 24. Basic Excision Tool fluorescent mode re-cuts.
13. Enter the well numbers of the missed pick-up spots in the "Re-cuts" box on the screen.
14. Click on "Begin/Resume" after the selections are made.
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