Bio-Rad Components for Older Model Spot Cutter - Manual (Page 4)

Section 1
Introduction
1.1 General Description
This manual describes the installation, operation, and maintenance of the Fluorescent Enclosure
for the ProteomeWorks Spot Cutter System. Use this manual in combination with the general
Spot Cutter manual that you received with your instrument. The Fluorescent Enclosure is
designed for use with the Bio-Rad ProteomeWorks Spot Cutter only.
The ProteomeWorks Spot Cutter with Fluorescent Enclosure is capable of accurately cutting
spots from gels and membrane blots with visible stains such as silver and coomassie, and with
fluorescent SYPRO Ruby protein stain. The system includes a 620/60 nm bandpass filter that is
optimized for fluorescent SYPRO Ruby protein stain.
1.2 Components Received
The ProteomeWorks Spot Cutter Fluorescent Enclosure, catalog number 165-7045, includes:
ProteomeWorks Fluorescent Enclosure
catalog number 165-7044
The Discovery Series software, catalog number 170-8605
(PDQuest version 6.2.1 or greater)
A new focussing / fluorescent imaging target has been added for use with the Fluorescent
Enclosure and the spot cutter. This target provides focussing lines for setting up the spot cutter
camera. It also provides fluorescent squares that can be imaged in the UV light mode. It is
suggested that this target be used after setting up the spot cutter and enclosure to test the cam-
era and lights before imaging a gel.
1.3 Common Re-order Parts
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Gel Sheets (thin plastic disposable sheets) 165-7018
Cutting Mat (thick plastic sheet - approx. 3 –4 mm) 165-7019
1.5 mm nominal Cutting Tip 165-7011
1.0 mm nominal Cutting Tip 165-7010
1.4 Physical Description
• The cabinet
• The door
• Quick release hinges
• Safety interlock switch
• Base mat
• UV-B lights
• 620 / 60 nm band pass filter
These parts are described in Figure 1.
1