Bio-Rad Components for Older Model Spot Cutter - Manual (Page 17)

1. When UV-Fluorescent imaging has been used on the spot cutter, at the end of the cut run
the software will prompt with, "Confirm cuts?"
Fig. 19. Confirm cuts window.
Answer "Yes" to bring up the following on-screen instructions for imaging the microtiter
plate in UV mode:
Fig. 20. Image Microtiter plate window.
2. Do not move the gel or membrane on the cutting platform
3. Place a clean plastic Gel Sheet on top of the gel
4. Place the microtiter plate on top of the plastic sheet. Note the orientation so that you can
recognize the A1 location.
5. Close the Enclosure door
6. Click on "Acquire image" when the plate is in place. The UV lights will turn on and the
camera will acquire an image with the same exposure time as the first spot cutter image.
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