Bio-Rad Components for Older Model Spot Cutter User Manual

Page 2

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Table of Contents

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1.1

General Description ..........................................................1

1.2

Components Received ....................................................1

1.3

Common Re-order Parts ..................................................1

1.4

Physical Description ........................................................1

1.5

Specifications ..................................................................2

1.6

Safety ..............................................................................2

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2.1

Unpacking the Enclosure..................................................3

2.2

Remove the Door ............................................................4

2.3

Installing the UV Lights ....................................................4

2.4

Installing the Bandpass Filter ............................................6

2.5

Spot Cutter Installation ....................................................7

2.6

Installing the Enclosure Cabinet........................................7

2.7

Software Setup ................................................................7

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3.1

Setting Aperture for White and UV Lights Settings ..........8

3.2

Toggle Switch for White or UV Lights ..............................9

3.3

Checklist for Operation in UV Light Mode ......................10

3.4

Checklist for Operation in White Light Mode ..................11

3.5

Calibration ......................................................................11

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4.1

Calibration and the UV Light Option ..............................12

4.2

Acquire Fluorescent Image in Basic Excision Tool ..........12

4.3

Cutting Spots From Fluorescent STained Gels in
Basic Excision Tool ........................................................13

4.4

Re-cuts in Basic Excision Tool........................................13

4.5

Begin/Resume and Pause Buttons ................................17

4.6

Confirm Cuts ..................................................................17

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5.1

Calibration and the UV Light Option ..............................18

5.2

Acquire Fluorescent Image in Basic Excision Tool ..........19

5.3

Re-cuts in Integrated Excision Tool ................................20

5.4

Begin/Resume and Pause Buttons ................................24

5.5

Confirm Cuts ..................................................................24

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