Bio-Rad Components for Older Model Spot Cutter - Manual (Page 28)

7. You should be able to see "holes" in the spots in the image within the overlay target circles.
However, there is a + or - 6% to 10% accuracy spec, so an edge of the "hole" may be
outside the circle and still be within specification.
Note:
The "snap to peak" and "gaussian fit" targeting features center the cuts on the densest
portion of the spot, not necessarily in the visual center of the spot. If the target circles and
cuts are off center, this does not necessarily mean that the targeting and cuts are off the
intended cut.
8. When you have viewed the cuts, click on the Begin/Resume button. This will close the new
image window with the overlays, and will prompt for loading the microtiter plate. Click OK
when you are ready to resume the cut run.
9. The cut run will resume on the next spot to be cut with the original cut targets.
10. You can repeat the "Confirm cuts" operation as many times as you want during the cut run.
The "confirm cuts" is an option in fluorescent gel cutting. Once you are familiar with targeting and
cutting fluorescent gels, you may or may not continue to use this function.
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