Positioning the gel – Bio-Rad Image Lab™ Software User Manual

Page 75

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Positioning the Gel

User Guide | 75

2.

To update an existing protocol, edit and then save your changes without
renaming the protocol.

3.

To create a new protocol:

a.

Edit the protocol.

b.

Choose File > Save As and type a different name.

Positioning the Gel

To position a gel

1.

Center the gel on the sample area.

Note: Ensure that the calibration area is clean and free of any dust or

droplets. Contamination can affect the calibration, and therefore the

results.

2.

Lower the lid.

3.

Set up a protocol or use an existing protocol.

4.

Click Preview Gel.

The densitometer quickly scans the sample gel. Based on the protocol settings,

Image Lab displays the results of the scan in a scan area box outlined in red as

the scan progresses.

Tip: If you determine that the settings are incorrect during the preview

scan (the sample of interest is not in the red box), click Cancel Run. Image

Lab returns to the Protocol Setup window where you can reset the scan

image coordinates.

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