Bio-Rad Bio-Plex® 200 Systems - Manual (Page 10)

2.2 Description of System Components
The Bio-Plex 200 system is comprised of the following components:
• Array reader — combines 2 lasers, fluidics, and real-time digital signal processing to distinguish
up to 100 different color-coded different color-coded bead sets, each representing a different
assay
• Microplate platform — automates the reading of 96-well plates, yielding up to 9,600 data
points in ~ 35 min
• PC and monitor — controls the Bio-Plex suspension array system via Bio-Plex Manager
software
• MCV (maintenance, calibration, and validation) plate IV — automates the maintenance,
calibration, and validation functions of the array reader
• Bio-Plex reservoir — allows maintenance functions to be seamlessly run before and after a
protocol resulting in improved walkaway capability
• Calibration kit — contains beads to standardize daily signal output and ensure unit-to-unit
reproducibility of the reader
• Validation kit — contains beads to validate the operational specifications of the reader,
including accuracy, linearity, dynamic range, slope, fluidics, and optical alignment
• Optional HTF — delivers up to 20 L of sheath fluid without user intervention
• Sheath fluid cube — contains 20 L of sheath fluid (1x) for the array reader
2.2.1 Array Reader
The array reader (Figure 1) is a compact flow analysis unit integrating a dual laser detection
system, optics, fluidics, and advanced digital signal processing. When used with the microplate
platform, the array reader facilitates the simultaneous analysis of up to 100 different analytes
from a single sample. The features of the array reader are outlined in Table 1.
Fig. 1. Array reader – front and side panel features.
5
www.bio-rad.com/bioplex/
Fluid connection
(side of reader)
Cheminert fitting
Sample arm
Sample needle
Needle height adjustment
thumbscrew
Access doors