Bio-Rad Bio-Plex® 200 Systems - Manual (Page 14)

2.2.3 High-Throughput Fluidics (HTF)
The Bio-Plex HTF (high-throughput fluidics) is designed to automate the introduction of sheath
fluid into the array reader (Figures 6 and 7). With the HTF, you can run samples continuously
without the need to replenish the sheath supply. The HTF automatically draws sheath fluid from
a nonpressurized bulk container to constantly maintain a reservoir of pressurized sheath fluid. A
single 20 L container provides enough sheath fluid for 48 hr or more of normal operation, or forty
96-well assay plates. The features of the HTF are outlined in Table 5.
Fig. 6. HTF – front view.
Fig. 7. HTF – rear panel view with connection to Bio-Plex 200 system.
Table 5. HTF Features.
Feature Description
Power indicator light Indicates that the power to the system is on
Fault indicator light Indicates when a fault has occurred with the system
Prime button Primes the HTF
Regulator adjustment screw Adjusts the sheath pressure of the array reader
Air in port Port where air line from array reader is connected
Sheath out port Port where sheath line from array reader is connected
Sheath in port Port where sheath supply (sheath cube) is connected to
the HTF
Communications port A DB9-PIN connector used to connect the HTF to the
reader
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Power indicator light
Fault indicator light
Prime button
Regulator adjustment screw
Air in port
Sheath out port
Sheath in port
Power connector
Communications
port P1
Communications
port P2 (to HTF)