Bio-Rad Bio-Plex® 200 Systems - Manual (Page 38)

Bio-Plex Manager has Most likely:
detected a change in
sheath pressure.
Sheath reservoir cap not Tighten sheath cap. Click OK.
on securely Message should disappear within 2
min.
Sheath bottle lines are not Make sure that all hoses are connected
connected properly to the appropriate ports, and that they
clicked into place.
Less likely:
Sheath fluid level above Adjust sheath fluid level so that sheath
the AIR port on the sheath fluid is below the AIR port of sheath
container bottle.
Sheath and waste bottle Make sure that the blue-ringed cap is
caps are reversed on the sheath bottle and the orange-
ringed cap is on the waste.
Sheath bottle has a leak Try new sheath bottle. Call technical
service for further assistance.
HTF unit not turned on Turn on HTF unit and ensure that
message disappears.
Air compressor not Listen for air pump to turn on when
working Warm Up is selected. Contact
Technical Support for further assistance.
Bio-Plex Manager has Room temperature has Calibrate array reader.
detected a change in the changed
temperature of the array
reader. Please calibrate
before running on assay to
ensure accurate results.
The calibration was Calibration procedure Make sure Cal1 beads and Cal2
unsuccessful. Please repeat failed beads are placed in the appropriate
calibration. If calibration wells (Cal1 in red well and Cal2 in
fails a second time, consult green well). Repeat calibration. Make
Troubleshooting Guide. sure you are using a clean MCV plate.
The calibration was Calibration procedure Run Unclog procedure, then repeat
unsuccessful. Bio-Plex failed due to low bead calibration. If problem persists, contact
Manager has detected a number Technical Support. See “low bead
problem with low bead number” in troubleshooting guide.
number. Please repeat
calibration.
Optical Validation Problem with optical Recalibrate the array reader and then
Procedure shows value(s) component of array repeat validation procedure. If values
outside of acceptable reader are still out of range, contact Technical
range(s). Support.
Reporter Validation Problem with optical Recalibrate the array reader and then
Procedure shows value(s) component of reader repeat validation procedure. If values
outside of acceptable are still out of range, contact Technical
range(s). Support.
Classify Validation Problem with calibration Recalibrate the array reader and then
Procedure shows value(s) or optical component of repeat validation procedure. If values
outside of acceptable reader are still out of range, contact Technical
range(s). Support.
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Message/Problem Causes Solution