Bio-Rad Bio-Plex® 200 Systems - Manual (Page 9)

1.2.3 Mechanical Safety Information
Caution: During operation, this system contains exposed, moving parts. Risk of personal injury
is present. Keep hands and fingers away from the sample probe and the syringe arm, as well
as the microplate platform during operation.
Note: Access doors must be closed while operating the Bio-Plex 200 system.
1.2.4 Biological Safety Information
Warning: All human and animal samples may contain hazardous infectious agents. Follow
appropriate biosafety procedures when handling these products and any containers.
Observe all local, state, and federal biohazard handling regulations when disposing of
biohazardous waste material.
1.2.5 Blue Indicator Light
Note: The blue lights above the sample arm, on the microplate platform, and on the
high-throughput fluidics (HTF) system indicate the on/off status of the respective system
components. The blue light emitting diode (LED) does not emit laser light or light in the UV
spectrum.
Section 2
Introduction
2.1 The Bio-Plex ® Suspension Array System and Multiplexing Technology
The Bio-Plex suspension array system is a unique and complete system comprising a 96-well
fluorescent microplate reader, Bio-Plex Manager
™ software, validation and calibration kits, and
assays. The system is designed, manufactured, and tested as a fully integrated system to
ensure accurate and reproducible assay results that are comparable across different laboratories.
Centered around a flow-based dual laser detector with real-time digital signal processing, the
Bio-Plex 200 system is able to distinguish up to 100 different families of color-coded, monodisperse
polystyrene beads, each bearing a different homogeneous capture assay (but all using the same
signal molecule) in a single 50
μl sample. This high degree of multiplexing dramatically increases
the amount of useful information from rare or volume-limited samples, such as mouse and rat
serum, and allows you to investigate analyte and biomarker interrelationships that would not
have been possible with traditional analysis systems. A microplate platform allows the automated
analysis of 96-well plates. The throughput of samples using this system will allow analysis of
more than 9,600 assay points in 30 min in a 96-well plate.
The Bio-Plex suspension array system uses up to 100 color-coded bead sets, each of which
may be conjugated with a unique specific reactant. Each reactant is specific for a different target
analyte. Reactants can include enzyme substrates, receptors, antigens, and antibodies to create,
for example, a capture sandwich immunoassay. To perform a multiplex assay, sample and
reporter molecules are allowed to react with the conjugated bead mixture in microplate wells.
The flow-based Bio-Plex 200 system identifies each specific reaction based on bead color and
quantitates it. The magnitude of the reaction is measured using fluorescently labeled reporter
molecules also specific for each target analyte. Bio-Plex Manager software automates data analysis
and generation of detailed summary reports. With the Bio-Plex suspension array system you can:
• Simultaneously quantitate up to 100 analytes per sample from culture media and serum
• Automatically analyze up to 96 samples in 30 min
• Instantly customize your assay by mixing Bio-Plex assays, or create your own assays
• Dramatically increase the amount of useful data obtained from a single sample
For more specific or updated information, visit us at www.bio-rad.com/bio-plex/
4
www.bio-rad.com/bioplex/