Bio-Rad Bio-Plex® 200 Systems - Manual (Page 35)

Section 5
Troubleshooting
5.1 Troubleshooting Guide for Bio-Plex ® 200 System
Problem Possible Cause Solution
Bio-Plex Manager™ Most Likely:
has detected a problem with
low bead number.
Too few beads in the assay Check bead number calculations.
Plate not shaken Remove plate from array reader
10 min before analysis and shake for 10 sec.
Buffer volume in wells is too Resuspend in 125 µl.
low (must be at least 125 µl) Perform Remove Bubbles.
Microbubble in cuvette Perform Remove Bubbles.
Perform Unclog to verify fluidics
integrity.
Low/no sheath fluid Refill sheath fluid, check sheath
connections. Perform Start Up.
Possible clog Perform Unclog and rerun. If
unsuccessful, repeat. Remove
needle (Figure 15) and sonicate in
cleaning solution or 10% bleach for
15 min. If still unsuccessful, contact
Technical Support.
Less Likely:
Incorrect needle height Adjust needle height.
Incompatible plate type used Replace with flat bottom or filter
plate and adjust needle height.
Vacuum system not Calibrate vacuum system.
calibrated
Red laser failure Contact technical service.
Filter plate not flat Check filter plate flatness.
Leaky filter plate 1. Check for liquid under plate on
microplate platform.
2. Check vacuum apparatus used
to prepare sample plate. Make
sure vacuum is below 5 in Hg.
3. Check needle height.
Incompatible suspension Check buffer compatibility.
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Message/Problem Causes Solution