Bio-Rad Bio-Plex® 200 Systems - Manual (Page 37)

Pressurizing in status bar of Leak in sheath bottle or Tighten sheath cap or replace sheath
software. cap bottle.
Sheath and waste bottle Make sure that the blue-ringed cap is
caps are reversed on the sheath bottle and the orange-
ringed cap is on the waste bottle.
System pressure settings Update pressure settings. Contact
incorrectly set Technical Support for instructions.
Needle stuck in down Protective assay plate See hardware manual for procedure for
position. covering was not removed raising needle stuck in down position.
Then remove cover from assay plate.
Needle guide is not Tighten needle guide by turning tube
screwed all the way in clockwise until tight.
Sample needle is bent Replace bent needle with a new
needle (Section 3.8.1, p. 16).
No assay signal detected. Most likely:
Error in assay preparation Make sure that streptavidin-PE was
added. See the assay kit manual.
Incorrect target values used Check that target values in calibration
in calibration dialog box match the values on Cal1
and Cal2 bottles.
Waste container overfilled Empty waste. Reconnect waste.
Perform Unclog.
Sheath reservoir low or Refill sheath reservoir. Perform
empty Start Up.
Less likely:
Waste line not connected Check waste line connection. Make
properly sure that it clicks into place. Make sure
cap is vented and there is no pressure
inside the waste bottle.
Laser not functioning Perform Calibrate using calibration kit.
Call Technical Support for further
assistance.
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Message/Problem Causes Solution
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