Bio-Rad Bio-Plex® 200 Systems - Manual (Page 3)

Table of Contents
Page
Section 1 General Information ......................................................................1
1.1 About This Manual ....................................................................................1
1.2 Safety Information .....................................................................................1
1.2.1 Electrical Safety Information ...........................................................2
1.2.2 Laser Safety Information ................................................................2
1.2.3 Mechanical Safety Information .......................................................4
1.2.4
Biological Safety Information ..........................................................4
1.2.5 Blue Indicator Light ........................................................................4
Section 2 Introduction ...................................................................................4
2.1 The Bio-Plex Suspension Array System and Multiplexing Technology .......4
2.2 Description of System Components ..........................................................5
2.2.1
Array Reader .................................................................................5
2.2.2 Microplate Platform ........................................................................8
2.2.3 High-Throughput Fluidics (HTF) .....................................................9
2.2.4 Computer and Monitor ..................................................................10
2.2.5 Maintenance, Calibration, and Validation Plate ............................10
2.2.6 Bio-Plex Reservoir .......................................................................10
2.3 Recommended Additional Equipment Not Provided ................................11
2.4 Bio-Plex Assays ......................................................................................11
Section 3 Installation ...................................................................................11
3.1 Unpacking ...............................................................................................11
3.2 System Location ......................................................................................11
3.3 Microplate Platform Setup .......................................................................12
3.4 Array Reader Setup .................................................................................12
3.5 Connecting the Sheath Fluid and Waste Containers ................................14
3.6 Computer and Monitor Connections ........................................................15
3.7 Software Installation ................................................................................15
3.7.1 System Software Loading ............................................................15
3.7.2 Communication Ports ..................................................................16
3.8 Installing or Changing the Sample Needle ...............................................16
3.8.1
Installing/Changing the Long Sample Needle ...............................16
3.8.2 Adjusting Sample Needle Height .................................................17
3.9 Initial System Priming...............................................................................19
3.10 Resetting Instrument Pressure Settings ..................................................20
3.11 High-Throughput Fluidics (HTF) Setup ....................................................20
3.12 Vacuum Manifold Setup ..........................................................................24
3.12.1 System Setup ..............................................................................24
3.12.2 Validation of Vacuum Pressure ....................................................25
3.13 Performing System Validation .................................................................26
Section 4 Care and Maintenance ................................................................27
Section 5 Troubleshooting Guide ...............................................................30
5.1 Troubleshooting Guide for Bio-Plex 200 System .....................................30
5.2 Troubleshooting Guide for Vacuum Manifold ...........................................35
5.3 Technical Support ...................................................................................36
Section 6 Bio-Plex 200 System Specifications ..........................................36
Section 7 Warranty Statement ....................................................................38
Section 8 Ordering Information – System Accessories ............................39
Section 9 Decontamination Information ....................................................41
Section 10 Legal Notices ..............................................................................42