Bio-Rad Rat Diabetes Assays - Manual (Page 13)

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4. Prepare Standards
General Instructions
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It is essential to prepare standards exactly as described in this section.
Incorrect preparation may lead to low signal or variable measurements
from plate to plate
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The peel-off sticker provided with the standards lists the most
concentrated point on the standard curve (S1). Enter this information
into Bio-Plex Manager
™ software as instructed in section 8
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For users who wish to mix assays from different panels, such as
diabetes assays with group I cytokines, guidance is provided here for
mixing 2 different lyophilized standards. Bead regions were chosen to
avoid overlap whenever possible. However, performance of multiplexes
containing assays from different groups have not been extensively
validated. Therefore, users must confirm that the assay performance is
still fit for their purpose
Selecting a Diluent for Standards
Refer to Table 5 for recommended diluents based on different sample types.
As a general rule, reconstitute and dilute standards in a diluent similar to
the final sample type or sample matrix.
Table 5. Summary of recommended diluents for standards.
Sample Type
Diluent for Standards Add BSA
Serum and plasma Standard diluent None
Culture media, with serum Culture media None
Culture media, serum-free Culture media To 0.5% final
For Adiponectin and/or Adipsin Assays
Serum and plasma
Serum-based diluent None