Bio-Rad Rat Diabetes Assays - Manual (Page 18)

16
5. Prepare Samples
General guidelines on preparing samples derived from serum, plasma,
and tissue culture supernatant are provided here, including the use of
protease inhibitors with plasma samples.
n
Once thawed, keep samples on ice. Prepare dilutions just prior to the
start of the assay and equilibrate to room temperature before use
n
Prepare sample dilutions in 1.5 or 2 ml polypropylene microcentrifuge
tubes. If a multichannel pipet will be used to load the plate, then aliquot
the required volumes into Titertube
® micro test tubes.
n
Do not freeze diluted samples
Table 8. Summary of recommended sample diluents and dilution factors.
Sample Type
Diluent Add BSA Sample Dilution
Serum and plasma Sample diluent None Fourfold (1:4)
Culture media, with serum Culture media None User optimized
Culture media, serum-free Culture media To 0.5% final User optimized
For Adiponectin Assay
Serum and plasma
Serum-based None Human (1:400)
diluent Mouse (1:1600)
NHP (1:1600)
For Adipsin Assay
Serum and plasma
Serum-based None Human (1:400)
diluent
Note: certain sample types may require a different dilution factor.