Bio-Rad Rat Diabetes Assays - Manual (Page 30)

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8. Read Plate
Bio-Plex Manager ™ software is recommended for all Bio-Plex Pro ™ assay
data acquisition and analysis. Instructions for Luminex xPONENT software
are also included. For instructions using other xMAP system software
packages, contact Bio-Rad Technical Support or your regional Bio-Rad
field applications specialist.
Prepare Protocol in Bio-Plex Manager
Software v 6.0 and Higher
The protocol should be prepared in advance so that the plate is read as
soon as the experiment is complete.
A protocol file specifies the analytes used in the reading, the plate wells
to be read, sample information, the values of standards and controls, and
instrument settings.
Bio-Plex Manager software version 6.0 contains protocols for most
Bio-Plex
® assays. Choose from available protocols or create a new
protocol. To create a new protocol, select File, then New from the main
menu. Locate and follow the steps under Protocol Settings.
1. Click Describe Protocol and enter information about the
assay (optional).
2. Click Select Analytes and create a new panel. Visually confirm the
selected analytes and proceed to step 3.
a. Click the Add Panel button in the Select Analytes toolbar.
Enter a new panel name. Select Bio-Plex Pro Assay Magnetic
from the assay pull-down menu. If using Bio-Plex Manager version
5.0 or lower, select MagPlex from the assay pull-down menu.
b. Click the Add button. Enter the bead region number and name
for the first analyte. Click Add Continue to repeat for each
analyte in the assay.
For reference, bead regions are shown in Table 20.