Bio-Rad Rat Diabetes Assays - Manual (Page 21)

19
Lavage, Sputum, and Other Biological Fluid Samples
Keep all samples on ice until ready for use.
1. If dilution is required, use Bio-Plex sample diluent with
0.5% BSA final.
2. Centrifugation at 10,000 x g for 10 min at 4°C may be needed to
clarify the sample.
Sample Dilution for Adiponectin and Adipsin Assays
Note: Physiological levels of adiponectin and adipsin are typically found
at high concentrations; therefore, higher sample dilutions are required to
achieve measurable concentrations within the standard curve.
1. For human adiponectin and adipsin assays, dilute serum or plasma
1:400 in serum-based diluent with two 1:20 serial dilutions
n
First dilution: 10 µl sample + 190 µl serum-based diluent
n
Second dilution: 10 µl from the first dilution + 190 µl serum-
based diluent
2. For mouse and NHP adiponectin assays, dilute serum or plasma
1:1,600 in serum-based diluent with two 1:40 serial dilutions
n
First dilution: 10 μl sample + 390 μl serum-based diluent
n
Second dilution: 10 μl from the first dilution + 390 μl serum-
based diluent