Bio-Rad Rat Diabetes Assays - Manual (Page 32)

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Table 21. Bead regions for compatible Bio-Plex Pro cytokine assays. Assays shown have
bead regions that are non-overlapping with diabetes assays.
3. Click Format Plate and format the plate according to the plate layout
created in Section 1 (Plan Plate Layout). To modify the plate layout,
follow the steps below (see Figure 6).
a. Select the Plate Formatting tab.
b. Select the standards icon
S
and drag the cursor over all
the wells that contain standards. Repeat this process for
blanks
B
, controls
C
, and samples
X
.
Human Cytokines Mouse Cytokines Rat Cytokines
Group I Bead Group II Bead Group I Bead Group II Bead Group I Bead
Region Region Region Region Region
IL-1b 39 IL-1a 63 IL-1a 53 IL-15 42 IL-1a 21
IL-1ra 25 IL-2Ra 13 IL-1b 19 IL-18 20 IL-1b 28
IL-2 38 IL-3 64 IL-2 36 Basic FGF 25 IL-2 22
IL-4 52 IL-12p40 28 IL-3 18 LIF 45 IL-4 33
IL-5 33 IL-18 42 IL-4 39 M-CSF 26 IL-5 52
IL-6 19 HGF 62 IL-5 52 MIG 44 IL-6 56
IL-7 74 IFN-a2 20 IL-6 38 MIP-2 27 IL-7 38
IL-8 54 LIF 29 IL-9 33 PDGF-BB 35 IL-10 19
IL-9 77 M-CSF 67 IL-10 56 VEGF 47 IL-12p40 76
IL-10 56 MIF 35 IL-12p40 76 IL-12p70 78
IL-12p70 75 b-NGF 46 IL-12p70 78 IL-13 15
IL-13 51 TNF-b 30 IL-13 37 IL-17 72
IL-15 73 TRAIL 66 IL-17 72 IL-18 20
IL-17 76 Eotaxin 74 EPO 14
Eotaxin 43 G-CSF 54 G-CSF 54
Basic FGF 44 GM-CSF 73 GM-CSF 37
G-CSF 57 IFN-g 34 GRO/KC 57
GM-CSF 34 KC 57 IFN-g 34
IFN-g 21 MCP-1
(MCAF)
51 M-CSF 26
IP-10 48 MIP-1a 77 MIP-1a 77
MCP-1 53 MIP-1b 75 MIP-2 27
MIP-1a 55 RANTES 55 MIP-3a 36
MIP-1b 18 TNF-a 21 RANTES 55
PDGF-BB 47 TNF-a 43
RANTES 37 VEGF 47
TNF-a 36 MCP-1 51
VEGF 45
* Mouse group III cytokines have not been tested for cross reactivity with diabetes or other panels.
Group III*
Bead
Region
IL-17F 28
IL-21 14
IL-22 15
IL-23p19 61
IL-25 67
IL-27p28 43
IL-31 29
IL-33 13
MIP-3a 12