Bio-Rad Rat Diabetes Assays - Manual (Page 27)

25
# of Wells
96
20x Detection
Antibodies, µl
150
Detection
Antibody
Diluent, µl
2,850
Total
Volume, µl
3,000
# of Wells
96
20x Detection
Antibodies, µl
Singleplex #1
150
20x Detection
Antibodies, µl
Singleplex #2
150
Detection
Antibody
Diluent, µl
2,700
Total
Volume, µl
3,000
# of Wells
96
20x Detection
Antibodies, µl
Diabetes
150
10x Detection
Antibodies, µl
Cytokines
300
Detection
Antibody
Diluent, µl
2,550
Total
Volume, µl
3,000
For reference, Table 15 summarizes volumes required for preparing
1x detection antibodies from a single 20x stock.
Table 16 summarizes volumes required for preparing 1x detection
antibodies from a mix of two 20x stocks; volumes required for
preparing detection antibodies of one diabetes (20x) assay and one
cytokine (10x) assay (for example, human insulin and human IL-6)
are listed in Table 17.
Preparing 1x detection antibodies from 20x stock (includes 25% excess volume).
Table 15. Premixed panel or one singleplex assay.
Table 16. Mixing two singleplex assays or a premixed panel + singleplex assay.
Table 17. Preparing 1x detection antibodies from two stocks at different concentrations.
Mixing human insulin (20x) with human IL-6 (10x) is one example.*
* Due to differences in dilution factors, it is not possible to multiplex adiponectin, adipsin
(human), VCAM-1, or ICAM-1 with other diabetes or cytokine assays.
5. After incubating the beads, samples, standards, and blank, slowly
remove and discard the sealing tape.