Bio-Rad Rat Diabetes Assays - Manual (Page 14)

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Assay Low RP1 (PMT) High RP1 (PMT)
Human/NHP* diabetes •
Mouse diabetes User validation required* •
Rat diabetes •
Low RP1 (PMT), broad High RP1 (PMT), narrow
Cross-panel mixing
range cytokine curve range cytokine curve
Human diabetes + cytokines •
Mouse diabetes + cytokines User validation required* •
Rat diabetes + cytokines •
Instrument RP1 (PMT)
Bio-Plex 100, 200* Low, high
Bio-Plex 3D* Standard, enhanced
Bio-Plex MAGPIX* N/A, use default instrument settings
* Or similar Luminex-based system.
RP1 (PMT) Setting for Standard Curves
The Bio-Plex 200 and 3D systems have two RP1 (PMT or photomultiplier
tube) setting options, while the Bio-Plex
® MAGPIX ™ has no PMT and
therefore no PMT setting options. Instead, MAGPIX uses default instrument
settings similar to low PMT on the Bio-Plex 200 (Table 6).
Table 6. Overview of PMT setting options on Bio-Plex systems.
The Bio-Plex Pro diabetes assays were developed on the high PMT
setting using the Bio-Plex 200 system. Protocols using alternative standard
dilution series or low PMT settings should be validated by the end user, for
example when mixing diabetes assays with cytokine assays (Table 7).
Table 7. Settings for optimal sensitivity on the Bio-Plex 200 system*.
* Contact Bio-Rad technical support for the most up-to-date recommendations on PMT
settings and cross-panel multiplexing compatibility.
Reconstitute a Single Vial of Diabetes Standards
This procedure prepares enough material to run each dilution in duplicate.
1. Gently tap the vial containing the lyophilized standard.
2. Add 500 μl of the appropriate standard diluent. Do not use assay
buffer or sample diluent to reconstitute the standards.