Bio-Rad Bio-Scale™ Mini CHT™ 40 µm Cartridges - Manual (Page 10)

Section 4
Preparing a Cartridge for
Use
Bio-Scale Mini CHT ceramic hydroxyapatite cartridges
are shipped dry. Prior to use, the following cartridge
conditioning steps should be followed:
1. Hold the cartridge vertically with the outlet in the
downward direction and then tap it against the
surface of the bench about 10 times.
2. Remove the luer caps and then attach to your
chromatography system with the outlet pointed
down (arrow pointing down).
3. It is recommended that the cartridge be
equilibrated with 25 ml of 1 N NaOH at 150 cm/hr
(2 ml/min).
4. Equilibrate the cartridge at 150 cm/hr (2 ml/min)
with 25–50 ml of 100 to 400 mM sodium phosphate
buffer, between pH 6.5 and pH 8.0.
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3.3 FPLC Systems
The luer to M6 adaptor fittings kit (catalog number
732-0111) provides fittings necessary to connect
the cartridge to the M6 fittings found on FPLC or
related systems.
Alternatively, connection can be made by using the
following Upchurch Scientific
® Quick Connect Luer
Adaptors: two Upchurch P-621 adaptor, 1/4–28, to
metric adaptors, one Upchurch P-619 adaptor,
1/4–28, to male luer, and one Upchurch P-628
adaptor, 1/4–28, to female luer. Assemble the luers
to the 1/4–28 metric adaptors. Attach the adaptor
with the male luer to the column inlet line of the
FPLC system and the one with the female luer to
the FPLC column outlet. To prevent tubing or
cartridge failure, do not exceed the maximum
recommended flow rate.
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