Bio-Rad Bio-Scale™ Mini CHT™ 40 µm Cartridges - Manual (Page 14)

Section 6
Technical Support
For additional information and technical support,
contact your local Bio-Rad office, or, in the US, call
1-800-4BIORAD (1-800-424-6723).
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5.2 Wash Alternatives
Perform wash alternatives with any of the following
alternative buffers in place of step 1. Continue with
step 2 listed in Section 5.1.
• 1–2 M KCI or NaCI
• 6 M urea
• 8 M guanidine-HCI
All the wash alternative buffers should contain 5 mM
phosphate at neutral pH.
5.3 Autoclaving
Bio-Scale Mini cartridges are not autoclavable.
5.4 Storage
After the cartridges are washed with deionized
water, Bio-Scale Mini CHT cartridges should be
purged and stored with 0.1 N NaOH. Higher
concentrations of NaOH may be used if desired.
Used CHT, after being regenerated and sanitized,
can be stored in up to 1.0 N NaOH at room
temperature.
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