Bio-Rad Bio-Scale™ Mini CHT™ 40 µm Cartridges User Manual
Page 13
Section 5
Care of the Cartridge
5.1 Cleaning
After repeated use, a CHT cartridge may require
thorough cleaning and regeneration to remove
bound contaminants. Most bound contaminants
may be removed by the following protocol:
Step 1. Wash the cartridge with 3–5 column
volume (5 ml/min for the 5 ml) of 500 mM
potassium or sodium phosphate, then
5 column volumes of starting buffer.
Step 2. Equilibrate the cartridge with at least
5 column volumes (5 ml/min for the 5 ml)
of starting buffer. If bound contaminants
persist after following the steps above, use
one of the alternative procedures listed in
wash alternatives.
19
C
CH
HT
T S
Su
uiittaab
biilliittyy
p
pH
H
B
Bu
uffffeerr
1
10
0 ccyycclleess
5
50
0 ccyycclleess
7.0
MES + 5 mM PO4
+
n/a
7.0
Acetate + 5 mM PO4
+
n/a
7.0
Imidazole + 5 mM PO4
+
+
7.0
Glycine + 5 mM PO4
+
n/a
7.0
Arginine + 5 mM PO4
+
n/a
7.0
HEPES + 5 mM PO4
+
n/a
7.0
Tris + 5 mM PO4
+
n/a
7.5
Phosphate (5 mM)
+
n/a
7.5
MES + 5 mM PO4
+
n/a
7.5
Imidazole + 5 mM PO4
+
+
7.5
Acetate + 5 mM PO4
+
n/a
7.5
HEPES + 2 mM PO4
+
n/a
7.5
HEPES + 5 mM PO4
+
n/a
7.5
Tris + 2 mM PO4
+
n/a
7.5
Tris + 5 mM PO4
+
+
8.5
Tris + 5 mM PO4
+
n/a
* All experiments performed in small scale columns. Each cycle used approximately 35 column
volumes of buffer to simulate an equilibration and long gradient, as well as five column volumes
of 1 N NaOH to simulate regeneration.
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