Bio-Rad Bio-Scale™ Mini CHT™ 40 µm Cartridges - Manual (Page 6)

Section 2
Connecting to Bio-Rad's
Low-Pressure
Chromatography
Instruments
The Bio-Scale Mini cartridges are ideal for use with
Bio-Rad's BioLogic
™ LP system, Econo ™ gradient
pump, and Model EP-1 Econo pump, and all low-
pressure chromatography instruments. Bio-Scale
Mini cartridges can be conveniently connected
directly to the system using the luer lock fittings on
the cartridge.
1. Install 1.6 mm ID tubing in the pumphead.
Adjust platen pressure screw (on pumphead).
—Using a screwdriver or coin, turn the screw
counterclockwise as far as it will go, then turn
clockwise three full turns. Assemble with
fittings and lock rings as shown in Figure 1.
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Table 2. Product Description
T Tyyp pee II T Tyyp pee IIII
FFu un nccttiio on naall g grro ou up pss Ca 2+ , PO 4 , OH Ca 2+ , PO 4 , OH
O Ob bsseerrvveed d d dyyn naam miicc b biin nd diin ng g ccaap paacciittyy
llyysso ozzyym mee ((L Lyyss)) ≥ 25 mg Lys/g CHT ≥ 12.5 mg Lys/g CHT
N No om miin naall p po orree d diiaam meetteerr 600–800 Е 800–1,000 Е
M Maaxxiim mu um m b baacckkp prreessssu urree 100 bar (1,500 psi) 100 bar (1,500 psi)
N No om miin naall m meeaan n p paarrttiiccllee ssiizzee 20 ± 2, 40 ± 4, and 80 ± 8 µm
B Bu ullkk d deen nssiittyy 0.63 g/ml 0.63 g/ml
O Ob bsseerrvveed d d dyyn naam miicc b biin nd diin ng g ccaap paacciittyy
IIg gG G 25–60 mg IgG/ml CHT* 15–25 mg IgG/ml CHT*
T Tyyp piiccaall lliin neeaarr ffllo ow w rraattee rraan ng gee 50–1,000 cm/hr
p pH H ssttaab biilliittyy 6.5–14
B Baassee ssttaab biilliittyy at least 21 months in 1 N NaOH
R Reeg geen neerraattiio on n 500 mM sodium phosphate, pH 7
1,000 mM trisodium phosphate, pH 11–12
A Au utto occllaavvaab biilliittyy ((b bu ullkk)) 121°C, 20 min in phosphate buffer, pH 7
S Saan niittiizzaattiio on n 1–2 N NaOH
R Reecco om mm meen nd deed d cco ollu um mn n sstto orraag gee 0.1 N NaOH
S Sh heellff lliiffee ((d drryy,, u un nu usseed d m maatteerriiaall)) 85 months stored dry, sealed, and at room
temperature
* 40 µm particles, 300 cm/hr, 5 mM sodium phosphate, pH 6.5
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