3 fplc systems – Bio-Rad Nuvia™ S Media User Manual

Page 16

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12

with a new piece of stainless steel tubing
attached to the union. Slip a short length of
0.8 mm ID tubing over

1

/

16

in OD stainless

steel tubing to a distance of 1 cm.

3.3 FPLC Systems

The luer to M6 adaptor fittings kit (catalog
#732-0111) provides fittings to connect a
cartridge to the M6 fittings found on FPLC or
related systems.

Alternatively, make a connection using
Upchurch Scientific adapters: two ¼–28
to metric adaptors (P-621), one ¼–28 to
male luer (P-619), and one ¼–28 to female
luer (P-628). Connect the luers to the ¼–28
metric adaptors. Attach the adaptor with
the male luer to the column inlet line of the
FPLC system and the adaptor with the female
luer to the FPLC column outlet. To prevent
tubing or cartridge failure, do not exceed the
maximum recommended flow rate.

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